Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), APC, eBioscience
마우스 CD1d를 인식하는 단클론 항체(1B1)로, APC 형광체가 결합된 제품입니다. 유세포 분석(Flow Cytometry)에 최적화되어 있으며, 0.25 µg/test로 사용 가능합니다. 4°C에서 암소 보관하며, 다양한 형광체 및 비결합 포맷으로도 제공됩니다.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.25 µg/test
- Publications: View 10 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1B1 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573134 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Biotin
- Brilliant Violet 480
- Brilliant Violet 711
- eFluor 450
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- NovaFluor Yellow 700
- NovaFluor Yellow 755
- PE
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein structurally homologous to MHC class I molecules. Although CD1d associates with β2-microglobulin like MHC I, its function is similar to MHC II. The 1B1 clone detects CD1d at varying levels on mouse leukocytes and recognizes β2m-associated CD1d.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test.
A test is defined as the amount of antibody required to stain cells in 100 µL. Optimal titration is recommended.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD1d is a heavy chain associated with β2-microglobulin on cortical thymocytes. It can also be expressed independently on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of MHC-like glycoproteins. The CD1d gene encodes a transmembrane glycoprotein that forms heterodimers with β2-microglobulin, mediating presentation of lipid and glycolipid antigens to T cells. The human genome contains five CD1 genes located on chromosome 1, differing in localization and ligand specificity. CD1d localizes to late endosomes and lysosomes via a tyrosine-based motif. Diseases associated with CD1D include Mycobacterium malmoense infection and autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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