Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• Publications: View 10 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Biotin
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein structurally homologous to MHC class I molecules. Although CD1d associates with β2-microglobulin like MHC I, its function is similar to MHC II. The 1B1 clone detects CD1d at varying levels on mouse leukocytes and recognizes β2m-associated CD1d.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test.
A test is defined as the amount of antibody required to stain cells in 100 µL. Optimal titration is recommended.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD1d is a heavy chain associated with β2-microglobulin on cortical thymocytes. It can also be expressed independently on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of MHC-like glycoproteins. The CD1d gene encodes a transmembrane glycoprotein that forms heterodimers with β2-microglobulin, mediating presentation of lipid and glycolipid antigens to T cells. The human genome contains five CD1 genes located on chromosome 1, differing in localization and ligand specificity. CD1d localizes to late endosomes and lysosomes via a tyrosine-based motif. Diseases associated with CD1D include Mycobacterium malmoense infection and autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지