Thermo Fisher Scientific CD50/ICAM3 Recombinant Rabbit Monoclonal Antibody (ICAM3, 2873R)
Recombinant rabbit monoclonal antibody targeting human CD50/ICAM3. Suitable for IHC (Paraffin) at 1–2 µg/mL. Expressed in HEK293 cells and purified via Protein A/G. Stored in PBS with BSA and sodium azide at 4°C. For research use only.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ICAM3, 2873R |
| Immunogen | Recombinant full-length human ICAM3 protein (CD50, UniProt ID: P32942-1, Antigen range: 1–547) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD50 (ICAM-3) is a transmembrane glycoprotein expressed by leukocytes and serves as a counter-receptor for the LFA-1 integrin. It mediates contact between T cells and antigen-presenting cells, regulates LFA-1 affinity for ICAM-1, and induces T cell activation and proliferation. ICAM-3 plays a key role in initiating immune responses and interacts with CD209 (DC-SIGN), facilitating communication between dendritic cells and granulocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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