Thermo Fisher Scientific CD50/ICAM3 Recombinant Rabbit Monoclonal Antibody (ICAM3, 2873R)
인간 ICAM3 단백질을 인식하는 재조합 토끼 단클론 항체. IHC(P) 및 억제 분석에 사용 가능. HEK293 세포에서 발현되며 Protein A/G로 정제됨. 보존제 무첨가 PBS 용액 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Inhibition Assays (IA) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ICAM3, 2873R |
| Immunogen | Recombinant full-length human ICAM3 protein (CD50, UniProt ID: P32942-1, amino acids 1–547) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
For formalin-fixed tissues, heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
CD50 (ICAM-3, intracellular adhesion molecule 3) is a transmembrane glycoprotein expressed by leukocytes. It functions as a counter-receptor for LFA-1 integrin and mediates adhesion between T cells and antigen-presenting cells. ICAM-3 regulates LFA-1 affinity for ICAM-1, promoting T cell activation and proliferation. It plays a critical role in initiating immune responses and interacts with CD209 (DC-SIGN), facilitating communication between dendritic cells and granulocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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