Thermo Fisher Scientific CD158a/h/g Monoclonal Antibody (HP-MA4), PE-Cyanine7, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• APC
• eFluor 450
• PE
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The monoclonal antibody HP-MA4 recognizes several CD158 proteins (KIR2D family), specifically KIR2DL1 (CD158a), KIR2DS1 (CD158h), KIR2DS3, and KIR2DS5 (CD158g).
It does not recognize KIR2DL2 (CD158b1), KIR2DS2 (CD158j), KIR2DL4 (CD158d), or KIR2DS4 (CD158i).
Crossreactivity to KIR2DL3 (CD158b2) and KIR2DL5 (CD158f) is unknown.

CD158 proteins are members of the killer cell immunoglobulin-like receptor (KIR) family expressed on NK cells and some T cells. Each KIR has a short (S) or long (L) cytoplasmic domain, mediating activating or inhibitory signaling, respectively.
Crossreactivity of HP-MA4 to KIR2DS3 and KIR2DS5 was reported in 2013.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.

Light Sensitivity: Tandem dye is sensitive to photo-induced oxidation. Protect from light.
Fixation: Samples can be stored in:

• IC Fixation Buffer (Product # 00-822-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Store up to 3 days at 4°C in the dark with minimal signal loss. Clone-specific performance should be verified empirically.

Excitation: 488–561 nm
Emission: 775 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by NK cells and subsets of T cells.
KIR genes are polymorphic, homologous, and located on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC).

KIR proteins are classified by:

• Number of extracellular immunoglobulin domains (2D or 3D)
• Cytoplasmic domain length (L or S)

KIR-L proteins transduce inhibitory signals via ITIM motifs.
KIR-S proteins lack ITIM and associate with TYRO binding protein to transduce activating signals.
Ligands are subsets of HLA class I molecules, contributing to immune regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지