Thermo Fisher Scientific ALK Monoclonal Antibody (OTI14E4), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Luminex (LUM)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Anaplastic Lymphoma Receptor Tyrosine Kinase (ALK) is part of the insulin receptor superfamily and plays a crucial role in brain development.
Mutations, rearrangements, and amplifications of the ALK gene are associated with various tumors, including anaplastic large cell lymphoma, neuroblastoma, and non-small cell lung cancer.
The most common alteration is chromosomal rearrangement, forming a fusion gene between ALK (chromosome 2) and nucleophosmin (NPM, chromosome 5).
The NPM-ALK fusion protein is oncogenic.
ALK encodes a receptor tyrosine kinase with transmembrane and extracellular domains, which are absent in the NPM-ALK product.
ALK shares high sequence similarity with LTK and functions in neuronal development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.