Thermo Fisher Scientific Histone H3ac (pan-acetyl) Polyclonal Antibody
Histone H3의 Lys9/14/18/23/27 아세틸화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. WB, IHC, ICC/IF, ChIP 등 다양한 응용 가능. 고순도 Affinity chromatography 정제. PBS + 50% glycerol 보존, -20°C 보관.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | – |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | – |
| ChIP assay (ChIP) | – | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human Histone H3 (Accession P68431), corresponding to amino acid residues T4–A25. Target family: H3ac pan-acetyl (K9, K14, K18, K23, K27). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899329 |
Product Specific Information
Antibody detects endogenous levels of Acetyl-Histone H3 only when acetylated at Lys9/14/18/23/27.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones can directly alter chromatin structure or facilitate the binding of specific transcription factors. The N-terminal tail of histone H3 can undergo various modifications such as methylation, acetylation, and phosphorylation, influencing numerous cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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