Thermo Fisher Scientific H4K12ac Polyclonal Antibody
H4K12ac에 특이적으로 결합하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫드 등 다양한 종에 반응하며, 액상 형태로 -20°C에서 보관. 아세틸화된 Lys12 위치의 Histone H4를 검출.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human Histone H4 (Accession P62805), corresponding to amino acid residues M1–Q28 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2899330 |
Product Specific Information
This antibody detects endogenous levels of Acetyl-Histone H4 only when acetylated at Lys12.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes. Two molecules each of H2A, H2B, H3, and H4 form an octamer, around which approximately 146 bp of DNA is wrapped to form nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and aids in chromatin compaction into higher-order structures. The gene encoding histone H4 is intronless and lacks polyA tails, containing instead a palindromic termination element. It is located within the large histone gene cluster on chromosome 6.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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