Thermo Fisher Scientific Phospho-PRKAR2A (Ser99) Recombinant Rabbit Monoclonal Antibody (SC54-04)
Phospho-PRKAR2A (Ser99) 인산화 단백질 검출용 재조합 토끼 단일클론 항체. Western blot, IHC, ICC/IF에 적합. 높은 특이성과 재현성 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC54-04 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser99 of Human PKA R2 (aa 82–126) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809490 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
cAMP is a signaling molecule crucial for various cellular functions. It activates the cAMP-dependent protein kinase (AMPK), which transduces signals via phosphorylation of target proteins.
The inactive holoenzyme of AMPK consists of two regulatory and two catalytic subunits. Upon cAMP binding, it dissociates into a dimer of regulatory subunits (bound to four cAMP molecules) and two free catalytic subunits.
This gene encodes one of the regulatory subunits, which can be phosphorylated by the activated catalytic subunit. It may interact with A-kinase anchoring proteins, influencing the subcellular localization of AMPK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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