Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience
Mouse IL-10 인식용 JES5-16E3 클론 단일클론 항체로 FITC 형광 표지되어 있음. Flow cytometry, ELISA, 중화 및 ICC/IF에 적합. 488 nm 여기/520 nm 방출 특성. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 35 publications |
| ELISA | - | View 1 publication |
| Neutralization | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JES5-16E3 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572513 |
Additional Formats Available:
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- Biotin
- Functional Grade
- PE
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10), an ~18 kDa cytokine also known as Cytokine Synthesis Inhibitory Factor (CSIF). IL-10 is produced by Th2 cells, B1 cells, macrophages, and keratinocytes, and inhibits Th1 cytokine synthesis and T cell proliferation. It acts as a costimulatory signal for mast cells, developing thymocytes, and the Th2 response.
Applications Reported:
- Capture of mouse IL-10 by ELISA and ELISPOT
- Intracellular staining for flow cytometry
- Immunohistochemistry (IHC)
- Neutralization of IL-10 bioactivity
Applications Tested:
Pre-titrated for intracellular staining and flow cytometric analysis of LPS-stimulated normal human peripheral blood cells. Recommended test size: 5 µL (0.25 µg)/test, suitable for 10⁵–10⁸ cells/test.
Optical Properties:
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
IL-10 encodes a cytokine primarily produced by monocytes and lymphocytes. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. IL-10 enhances B cell survival, proliferation, and antibody production, blocks NF-kappa B activity, and modulates the JAK-STAT signaling pathway. Knockout studies in mice indicate its essential role in intestinal immunoregulation. Mutations in IL10 are associated with increased susceptibility to HIV-1 infection and rheumatoid arthritis. Related diseases include Graft-Versus-Host Disease and HIV-1 infection.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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