Thermo Fisher Scientific NFkB p65 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 6 publications 6 publications

Flow Cytometry (Flow)

3-5 µg/1x10^6 cells

-

Immunoprecipitation (IP)

3 µg

-

ChIP assay (ChIP)

1-5 µg x 10^6 cells

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 30% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539917

Product Specific Information

PA1-186 has been successfully used in WB using StartingBlock T20 (TBS) Blocking Buffer (Product # 37543). More non-specific bands are observed when using 5% BSA for blocking.

By WB, PA1-186 detects a predominant band at 65kD. Loading increasing amounts of cell lysate may yield more intense nonspecific bands at ~120kD and/or ~50kD.

PA1-186 has been succesfully used for IP of the NF-kB p65 subunit and co-IP of the NF-kB p50 subunit.

Target Information

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.