Thermo Fisher Scientific E6AP Polyclonal Antibody
E6AP(UBE3A) 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, Flow Cytometry에 사용 가능. 고순도 액상 형태(2.0 mg/mL)로 제공되며, PBS buffer에 sodium azide 포함. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between amino acids 836–865 from the C-terminal region of human UBE3A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.0 mg/mL |
| Purification | Size-exclusion – Dialysis |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2211786 |
Target Information
UBE3A (ubiquitin-protein ligase E3A, also known as E6AP)
UBE3A is an E3 ubiquitin-protein ligase that transfers ubiquitin from an E2 enzyme to its substrates. Known substrates include ARNTL/BMAL1, ARC, RAD23A, RAD23B, MCM7, annexin A1, PML tumor suppressor, and CDKN1B.
UBE3A may also function in cellular quality control by aiding degradation of misfolded cytoplasmic proteins and plays a role in circadian rhythm regulation.
Defects in UBE3A are associated with Angelman syndrome (AS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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