Thermo Fisher Scientific IFN gamma Recombinant Rabbit Monoclonal Antibody (11H20L4)

Applications

• Western Blot (WB): Tested dilution 1–3 µg/mL

Product Specifications

Product Specific Information

This antibody is predicted to react with non-human primate, mouse, and rat based on sequence homology.
Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages:

• High specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity due to large rabbit immune repertoire

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and natural killer cells in response to antigens and cytokines.
It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits. On SDS-PAGE, IFN gamma appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.

Functions include:

• Antiviral and tumor antiproliferative activity
• Induction of MHC class I and II
• Macrophage activation
• Enhanced immunoglobulin secretion by B lymphocytes

The biological activity of IFN gamma is species specific; human IFN gamma does not cross-react with mouse.
Activation occurs through IFN gamma receptor I and II, triggering the JAK-STAT pathway.
Human IFN gamma shares ~40% sequence homology with mouse IFN gamma.

Regulation:
Upregulated by IL2, FGF basic, and EGF; downregulated by vitamin D3 or DMN.
Mutations in the IFN gamma gene are associated with aplastic anemia.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.