Thermo Fisher Scientific Phospho-MEK1 (Ser217, Ser221) Recombinant Rabbit Monoclonal Antibody (13H22L22)
Recombinant rabbit monoclonal antibody recognizing phospho-MEK1 (Ser217/Ser221). Validated for WB and ChIP. High specificity and consistency with animal-free formulation. Suitable for studying MAPK pathway activation and MEK1 phosphorylation.
- 카탈로그번호
- 701267
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-MEK1 (Ser217, Ser221) Recombinant Rabbit Monoclonal Antibody (13H22L22)
Applications
Western Blot (WB)
- Tested Dilution: 1–3 µg/mL
ChIP assay (ChIP)
- Tested Dilution: 1 µL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 13H22L22 |
| Immunogen | Phosphopeptide corresponding to amino acids 212–226 of human MAP2K1 [pSpS217/221] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532449 |
Product Specific Information
- Predicted to react with mouse, rat, Drosophila, non-human primate, and rabbit based on sequence homology.
- Intact IgG appears on a non-reducing gel as a ~150 kDa band, and upon reduction generates ~25 kDa light chain and ~50 kDa heavy chain bands.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning antibody DNA sequences from immunoreactive rabbits.
- Advantages include better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity due to the larger rabbit immune repertoire.
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase functioning in MAPK cascades, regulating cell growth and differentiation. It is activated by various growth factors, cytokines, membrane depolarization, and calcium influx. MEK1 activation occurs via phosphorylation of serine residues 218 and 222 by Raf1. It plays roles in stress response, apoptosis, and cytokine-induced proliferation. Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.