Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Recombinant Rabbit Monoclonal Antibody (9H12L10)
Phospho-Histone H3 (Ser10)을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, ICC, ChIP 등 다양한 응용에 적합. 높은 특이성과 재현성을 제공하며, lot 간 일관성이 우수. PBS 버퍼 내 0.5 mg/mL 농도로 제공되며 4°C 또는 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–3 µg/mL | - |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 0.05–2 µg | - |
| ChIP assay (ChIP) | 3 µg | - |
| Peptide Array (Array) | 0.25 µg/mL | - |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Nematode |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 9H12L10 |
| Immunogen | Phosphopeptide corresponding to amino acids 4–16 of human Histone H3 [pS10] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532444 |
Product Specific Information
This antibody is predicted to react with mouse, rat, non-human primate, and rabbit based on sequence homology.
- Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction generates a ~25 kDa light chain and a ~50 kDa heavy chain.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits.
- Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it forms nucleosomes that compact DNA.
Histones are essential for transcription regulation, DNA repair, replication, and chromosomal stability. The N-terminal tail of histone H3 undergoes various post-translational modifications such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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