Thermo Fisher Scientific alpha Actinin 2 Recombinant Rabbit Monoclonal Antibody (7H1L69)
Recombinant rabbit monoclonal antibody targeting alpha Actinin 2. Validated for WB, IHC, ICC/IF, and IP. High specificity and lot-to-lot consistency. Suitable for human, mouse, and rat samples. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 2 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 1:100 | View 3 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7H1L69 |
| Immunogen | Peptides corresponding to human Sarcomeric alpha actinin/alpha actinin 2 (aa 5–24 and aa 834–852) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2688290 |
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Rat, and Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences from immunoreactive rabbits are cloned and expressed, then screened to select optimal clones.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal-origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
Alpha actinins belong to the spectrin gene superfamily, a group of cytoskeletal proteins including alpha/beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles:
- In nonmuscle cells: localizes to microfilament bundles and adherens junctions, linking actin to membranes
- In muscle cells: found in Z-discs and dense bodies, anchoring myofibrillar actin filaments
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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