Thermo Fisher Scientific PD-L2 (PDCD1LG2) Monoclonal Antibody (OTI8A4), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Flow Cytometry (Flow)

• Tested Dilution: Assay-Dependent

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Programmed death-ligand 2 (PD-L2, also known as B7-DC) is a member of the B7 ligand family within the immunoglobulin superfamily. Along with PD-L1, it acts as a ligand for PD-1. PD-L2 is primarily expressed in dendritic cells but can be induced in various immune and non-immune cells depending on the microenvironment.

PD-L2 expression increases in response to Th2 cytokine IL-4 and to a lesser extent by Th1 cytokines TNF-alpha and IFN-gamma. Although expressed at lower levels than PD-L1, PD-L2 has a 2–6 times higher affinity for PD-1. PD-1 and its ligands serve as inhibitory immune checkpoint molecules, regulating T cell activation, proliferation, and cytokine secretion (e.g., IFN-gamma, IL-10).

Interestingly, PD-L2 can also stimulate T cell proliferation and cytokine production independently of PD-1, suggesting additional receptors. Recent studies identified repulsive guidance molecule b (RGMb) as a second receptor for PD-L2. RGMb, expressed in the CNS and macrophages, contributes to respiratory tolerance through BMP and/or neogenin signaling pathways.

The human PD-L2 monomer includes:

• 201 amino acid extracellular domain
• 21 amino acid transmembrane domain
• 32 amino acid cytoplasmic domain

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.