Thermo Fisher Scientific SARS-CoV/SARS-CoV-2 NP Recombinant Human Monoclonal Antibody (CR3009 (03-009))
SARS-CoV 및 SARS-CoV-2 N 단백질을 인식하는 인간 재조합 단일클론 항체입니다. ELISA 및 ICC/IF 등 다양한 면역분석에 사용됩니다. HEK293 세포에서 발현되었으며, Protein A로 정제된 액상 형태입니다. 연구용으로만 사용 가능합니다.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: Assay-dependent
ELISA
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Human / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | CR3009 (03-009) |
| Immunogen | The original antibody was generated by cloning the variable regions of the scFvs selected from phage display libraries into separate vectors for IgG1 heavy-chain and light-chain expression. The harvested supernatants were then purified on protein A columns. The original antigen was the whole irradiated virion. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_3074768 |
Product Specific Information
This antibody recognizes and binds the non-linear/conformational epitope of the N protein of SARS-CoV and also binds the SARS-CoV-2 nucleoprotein.
Target Information
Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses with large RNA genomes (27–31 kb). The first step in coronavirus infection is binding of the viral spike protein (139 kDa) to receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants of SARS virus-infected cells is a 46 kDa nucleocapsid protein, suggesting that this protein is a major immunogen useful for early diagnostics. SARS-CoV-2 Nucleocapsid Protein is associated with nucleic acid and is the most abundant coronavirus protein. Due to its strong immunogenicity, it has potential value for diagnostic applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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