Thermo Fisher Scientific BRD4 Chimeric Recombinant Rabbit Monoclonal Antibody (RAB-C131)
BRD4 단백질을 인식하는 재조합 토끼 단클론 항체. Western blot, ICC/IF, Flow cytometry 등 다양한 응용에 적합. HEK293 세포에서 발현된 IgG kappa 항체로, Protein A 정제 및 PBS buffer에 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 0.03 µg/mL | |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | |
| Flow Cytometry (Flow) | 1:100 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent | |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RAB-C131 |
| Immunogen | Recombinant antibody (rAb) phage display recognizing BRD4 protein under non-denaturing conditions |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074770 |
Product Specific Information
Specificity: This antibody recognizes BRD4 (Bromodomain-containing protein 4). It binds to a folded domain, amino acids 333–460. BRD4 is a chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory.
Target Information
BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of the BET protein family. It is a human homolog of mouse MCAP and consists of two N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as two isoforms; the longer variant has a C-terminal region with proline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. It acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also plays a role in regulating chromosomal dynamics during mitosis and functions as a cellular target for the E2 protein of papillomavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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