Thermo Fisher Scientific Plexin-A1 Monoclonal Antibody (M535)
Plexin-A1 단백질을 인식하는 마우스 단클론 항체로, WB, IHC, ICC, IP 등 다양한 응용에 사용 가능. 인간, 생쥐, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체이며, 세포 이동과 축삭 유도 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M535 |
| Immunogen | Clone M535 was generated from a Plexin-A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the N-terminal half of the semaphorin domain in human Plexin-A1. This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | ~1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942660 |
Product Specific Information
This antibody detects a 220 kDa* protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of neonatal mouse brain, and detects a recombinant mouse Plexin-A1 protein containing the extracellular region.
Target Information
Coreceptor for SEMA3A, SEMA3C, SEMA3F, and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth, and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for activation of downstream signaling events in the cytoplasm. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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