Thermo Fisher Scientific CD100 (SEMA4D) Monoclonal Antibody (M188)
CD100(SEMA4D) 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot 및 Immunocytochemistry에 적합. 인간, 마우스, 랫트 시료에 반응하며, 뇌 조직에서 150 kDa 밴드를 검출. Protein G 정제, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M188 |
| Immunogen | Clone (M188) was generated from a recombinant protein corresponding to the C-terminal region of human Semaphorin-4D. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942661 |
Product Specific Information
This antibody detects a 150 kDa* band on SDS-PAGE immunoblots of adult mouse brain.
Target Information
Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development.
CD100 (also known as semaphorin 4D), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro.
CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and downregulating CD23 expression.
CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling.
CD100 is expressed on resting and PHA-stimulated T cells. The protein is weakly expressed on NK cells, EBV-transformed B cells, monocytes, and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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