Thermo Fisher Scientific VASP Monoclonal Antibody (M277)
VASP 단백질을 인식하는 Mouse monoclonal 항체(M277)로, Human 시료에 반응합니다. WB(1:50) 및 ICC/IF(1:2000)에 사용 가능합니다. Protein A 정제, PBS/BSA/glycerol buffer에 보관되며, -20°C에서 안정적으로 저장됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M277 |
| Immunogen | Clone (M277) was generated from a recombinant protein that includes amino acids from the C-terminal region of human VASP. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.0625 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942665 |
Product Specific Information
This antibody detects 46 and 50 kDa proteins corresponding to the apparent molecular mass of VASP on SDS-PAGE immunoblots of human A431, HeLa, and HUVEC.
Target Information
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin.
VASP is associated with filamentous actin formation and plays a role in cell adhesion and motility. It may also be involved in intracellular signaling pathways regulating integrin-extracellular matrix interactions. VASP is regulated by cyclic nucleotide-dependent kinases PKA and PKG.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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