Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Yellow 660, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Yellow 660

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test

---

Product Specifications

Additional Formats Available:
Unconjugated, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 661/737/805, Brilliant Violet 480, eFluor 450/506, FITC, Functional Grade, NovaFluor Blue/Red/Yellow variants, PE, PE-Cyanine5/7, PE-eFluor 610, PerCP-eFluor 710, Super Bright 436/600/645/702/780, Custom conjugation available.

---

Product Specific Information

The eBioCB16 monoclonal antibody recognizes human CD16 (Fc gammaRIII), a low-affinity receptor for IgG (50–80 kDa).
CD16 exists as two forms:

• CD16A (FcγRIIIA): Found on macrophages and NK cells (hetero-oligomeric, polypeptide-anchored)
• CD16B (FcγRIIIB): Found on neutrophils (monomeric, GPI-anchored)

Two polymorphisms, NA-1 and NA-2, affect phagocytic capacity. CD16 binds IgG immune complexes (preferentially IgG1, IgG3) and initiates signaling cascades leading to ADCC, phagocytosis, degranulation, and proliferation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells.
Use 5 µL (0.4 µg) per 100 µL of cell suspension (10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• Always include CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) in antibody cocktails for optimal staining.
  • Add 5 µL per sample (regardless of number of NovaFluor dyes).
  • For single-color controls, use 5 µL per 100 µL of cell sample.

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on engineered nucleic acid dye structures for optimized fluorescence with reduced spillover and spread.

• Excitation: 552 nm
• Emission: 663 nm
• Laser: 561 nm (Yellow)

Learn more about NovaFluor dyes

---

Target Information

CD16 (FcγRIII) encodes a receptor that binds the Fc portion of IgG, mediating immune complex clearance and cellular cytotoxicity.

• Gene: FCGR3A
• Location: Chromosome 1
• Expression: NK cells (transmembrane form), PMNs (PI-linked form)
• Associated Diseases: Immunodeficiency 20, Herpes Zoster, Systemic Lupus Erythematosus, Alloimmune Neonatal Neutropenia

Alternative splicing results in multiple isoforms. Mutations in FCGR3A may increase susceptibility to recurrent viral infections.

---

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

---

제품 이미지