Thermo Fisher Scientific CD319 (CRACC) Monoclonal Antibody (162), PE, eBioscience
CD319(CRACC) 단일클론 항체(클론 162), PE 형광 표지로 인간 세포 분석용. Flow Cytometry에 최적화되어 있으며, SAP 독립적 신호전달 경로 연구에 적합. 4°C 암소 보관, 동결 금지. 연구용으로만 사용 가능.
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Thermo Fisher Scientific CD319 (CRACC) Monoclonal Antibody (162), PE, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (1 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 162 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11039393 |
Product Specific Information
The monoclonal antibody 162 recognizes CRACC (CD2-like receptor for activating cytotoxic cells), also known as CD319 and CS1, a member of the SLAM receptor family.
CRACC is a 66 kDa glycosylated transmembrane protein containing a cytoplasmic ITSM domain (Immunoreceptor Tyrosine-based Switch Motif).
Unlike other SLAM family members, CRACC’s ITSM domain is SAP-independent and instead uses EAT-2 as a signaling molecule. This feature is significant for patients with X-linked lymphoproliferative disease (SAP mutation).
A splice variant lacking the ITSM domain has been reported.
Applications Reported:
The 162 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Recommended usage: 5 µL (1 µg) per test (final volume 100 µL).
Cell number: 10⁵–10⁸ cells/test (empirically determined).
Excitation/Emission:
488–561 nm / 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
SLAMF7 contains one Ig-like C2-type domain.
Isoform 1 mediates NK cell activation through a SAP-independent ERK-mediated pathway and may play a role in lymphocyte adhesion.
Isoform 3 does not mediate activation.
SAP binds the cytoplasmic tail of isoform 1 when phosphorylated in the presence of Fyn (in vitro).
SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung, and trachea.
Detected in NK cells, activated B cells, and NK-cell lines; not in promyelocytic, B-, or T-cell lines.
Isoform 3 is expressed at much lower levels than isoform 1.
There are three named isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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