Thermo Fisher Scientific CD213a1 (IL-13Ra1) Monoclonal Antibody (13MOKA), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

13MOKA

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10667742

Product Specific Information

Description: The 13MOKA monoclonal antibody reacts with mouse CD213 alpha 1 (interleukin-13 receptor alpha 1, IL-13Ra1). CD213a1 is the low-affinity IL-13 receptor which mediates normal IL-13 inflammatory signaling. It has been shown that CD213a1 binds to IL-13 which then recruits CD124 (IL-4Ralpha) to stabilize the low-affinity interaction and form the active signaling complex. The CD213a1/CD124 complex can also serve as a receptor for IL-4, although the role of CD213a1 in this complex is poorly understood. CD213a1 is expressed on activated B and T cells and other cells.

Applications Reported: This 13MOKA antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 13MOKA antibody has been tested on mouse splenocytes stimulated for 3 days with Anti-CD3 and Anti-CD28 Functional Grade antibodies. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.