Thermo Fisher Scientific CD209 (DC-SIGN) Monoclonal Antibody (eB-h209), PE, eBioscience
CD209 (DC-SIGN) 단백질을 인식하는 인간 특이적 Rat IgG2a 단클론 항체로, PE 형광 표지되어 유세포분석(Flow Cytometry)에 최적화됨. HIV 및 ICAM-3 결합 연구에 유용하며, 4°C 암소 보관 권장.
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Thermo Fisher Scientific CD209 (DC-SIGN) Monoclonal Antibody (eB-h209), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 7 publications |
| Immunomicroscopy (IM) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eB-h209 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10853324 |
Additional Formats
Product Specific Information
The eB-h209 monoclonal antibody reacts with human CD209 (DC-SIGN), a 44 kDa type II transmembrane protein containing a C-type lectin binding domain that binds ICAM-3, ICAM-2, and HIV virus. DC-SIGN is preferentially expressed on human dendritic cells and may function as a receptor for HIV capture and transmission to T cells, supporting primary immune response.
This antibody was developed against a C-terminal peptide of human DC-SIGN.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated for flow cytometric analysis of cultured human dendritic and peripheral blood cells. Recommended use: 5 µL (0.06 µg) per test (100 µL total volume).
- Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD209 (DC-SIGN) encodes a transmembrane receptor expressed on dendritic cells and macrophages, functioning in innate immunity by recognizing diverse pathogens including parasites and viruses. The protein has three domains: an N-terminal transmembrane domain, a tandem-repeat neck domain, and a C-type lectin carbohydrate recognition domain.
The extracellular region acts as both a pathogen recognition and cell adhesion receptor. The neck region facilitates homo-oligomerization for multivalent ligand binding.
Variants in the neck domain affect ligand binding affinity. CD209 is closely related to L-SIGN (GeneID 10332), differing in ligand-binding properties and tissue distribution. Alternative splicing produces multiple variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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