Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), PE, eBioscience
마우스 CD223(LAG-3)을 인식하는 단클론 항체로, 활성화된 T세포와 NK세포의 분석에 적합. PE 형광 표지로 유세포분석(Flow Cytometry)에 최적화. 0.5 µg/test 권장. 4°C 암소 보관, 동결 금지. 연구용으로만 사용 가능.
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카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), PE, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.5 µg/test
- Publications: 20 publications available
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioC9B7W (C9B7W) |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_494216 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Brilliant Violet 480
- eFluor 450
- FITC
- Functional Grade
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702
- Custom conjugation available
Product Specific Information
The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated α/β-TCR-bearing T cells, a subset of γ/δ-TCR-bearing T cells, and NK cells. CD223 is a 70 kDa type I transmembrane protein similar to CD4. A soluble form of CD223 can be generated by proteolytic cleavage, resulting in a 54 kDa extracellular fragment and a 16 kDa intracellular fragment.
CD223 binds MHC class II with higher affinity than CD4, participating in negative regulation of T-cell activation and homeostatic proliferation. It is expressed on CD4⁺CD25⁺ regulatory T cells and may contribute to their suppressive function.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on stimulated mouse splenocytes
- Recommended ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
- Optimize titration for best performance
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
LAG-3 is a 70 kDa surface glycoprotein of the Ig superfamily with homology to CD4. It binds MHC class II with higher affinity than CD4 and is involved in negative regulation of T-cell activation and proliferation. LAG-3 is expressed on activated T cells (including regulatory T cells) and NK cells, with CD8⁺ T cells showing higher expression than CD4⁺ T cells. Coexpression of LAG-3 and CD49b can identify Type 1 regulatory T cells (Tr1 cells) in humans and mice.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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