Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: 20 publications available

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant Violet 480
• eFluor 450
• FITC
• Functional Grade
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702
• Custom conjugation available

Product Specific Information

The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated α/β-TCR-bearing T cells, a subset of γ/δ-TCR-bearing T cells, and NK cells. CD223 is a 70 kDa type I transmembrane protein similar to CD4. A soluble form of CD223 can be generated by proteolytic cleavage, resulting in a 54 kDa extracellular fragment and a 16 kDa intracellular fragment.

CD223 binds MHC class II with higher affinity than CD4, participating in negative regulation of T-cell activation and homeostatic proliferation. It is expressed on CD4⁺CD25⁺ regulatory T cells and may contribute to their suppressive function.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on stimulated mouse splenocytes
  • Recommended ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
  • Optimize titration for best performance
• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

LAG-3 is a 70 kDa surface glycoprotein of the Ig superfamily with homology to CD4. It binds MHC class II with higher affinity than CD4 and is involved in negative regulation of T-cell activation and proliferation. LAG-3 is expressed on activated T cells (including regulatory T cells) and NK cells, with CD8⁺ T cells showing higher expression than CD4⁺ T cells. Coexpression of LAG-3 and CD49b can identify Type 1 regulatory T cells (Tr1 cells) in humans and mice.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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