Thermo Fisher Scientific Phospho-STAT5 alpha (Tyr694) Recombinant Rabbit Monoclonal Antibody (6H5L15)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Intact IgG appears on a non-reducing gel as a ~150 kDa band and, upon reduction, generates a ~25 kDa light chain band and a ~50 kDa heavy chain.
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits.
• Individual clones are screened to select the best candidates for production.
• Advantages include:
  • Improved specificity and sensitivity
  • Lot-to-lot consistency
  • Animal origin-free formulations
  • Broader immunoreactivity to diverse targets

Target Information

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by receptor-associated kinases, forming homo- or heterodimers that translocate to the nucleus and act as transcription activators.

STAT5 alpha is activated by ligands such as IL2, IL3, IL7, GM-CSF, erythropoietin, thrombopoietin, and various growth hormones. Activation in myeloma and lymphoma associated with TEL/JAK2 fusion is independent of cell stimuli and essential for tumorigenesis.

In mice, STAT5 alpha induces expression of BCL2L1/BCL-X(L), suggesting an anti-apoptotic role. STAT5 alpha and STAT5 beta share 96% sequence similarity and are co-expressed in most tissues, though their mRNA levels differ in muscle and mammary tissues.

STAT5 alpha is critically involved in reproduction, lactation, immune function, and somatic growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.