Thermo Fisher Scientific CD11b Monoclonal Antibody (5C6)
CD11b 단백질을 인식하는 Rat IgG2b 단일클론 항체로, 인간 및 마우스 시료에 반응합니다. Flow cytometry, IHC, ICC, IP 등 다양한 응용에 사용 가능하며, Protein G로 정제된 액상 형태입니다. 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC-F) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | 1:100 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5C6 |
| Immunogen | Thioglycollate-elicited Peritoneal Macrophages (TPM) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C (short term); -20°C (long term). Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538032 |
Additional Formats
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Rat anti-Mouse CD11b antibody, clone 5C6 recognizes CD11b, also known as the integrin alpha M chain.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 complex (integrin alpha-M beta-2, Mac-1, CR3) acts as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
CD11b is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, subsets of NK and dendritic cells, and microglia. Expression increases during monocyte maturation and varies among tissue macrophages.
Peritoneal macrophages express higher CD11b levels than splenic macrophages. CD11b dysfunction is associated with systemic lupus erythematosus and ITGAM-related susceptibility to autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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