
Thermo Fisher Scientific Phospho-ERK1/ERK2 (Tyr204) Polyclonal Antibody
ERK1/ERK2 인산화(Tyr204) 부위를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF 등 다양한 응용 가능. 인간, 마우스, 랫트 반응성. 고순도(>95%) 항원 친화 크로마토그래피 정제. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Tyrosine 204 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553723 |
Product Specific Information
- Detects endogenous protein at molecular weights of 42 and 44 kDa
- Purity >95% by SDS-PAGE
Target Information
ERK1 and ERK2 are widely expressed and involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
They are activated by various stimuli, including growth factors, cytokines, virus infection, G protein-coupled receptor ligands, and transforming agents.
Upon activation, ERKs phosphorylate cytoplasmic targets or migrate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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