Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), Brilliant Ultra Violet 395, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 563, 615, 661, 737
• Brilliant Violet 480, 605, 650, 711
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells throughout development except plasma cells. Follicular dendritic cells also express CD19. Together with CD21, CD81, Leu13, and MHC class II, CD19 forms a multimolecular complex associated with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation.
HIB19 overlaps with SJ25C1 in epitope recognition.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm, suitable for cytometers with UV (355 nm) laser. Ensure instrument compatibility.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and some myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the B cell receptor (BCR) and regulates proliferation and differentiation.
Mutation of CD19 leads to hypogammaglobulinemia, while overexpression causes B cell hyperactivity.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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