Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), Brilliant Ultra Violet 395, eBioscience

Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 615
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 610-30S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645

Product Specific Information

The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage. CD24 is variably glycosylated, resulting in heterogeneity of molecular mass across different cell lineages. Expression of CD24 helps resolve stages of B lymphopoiesis in mouse bone marrow, and P-selectin (CD62P) has been reported to bind to CD24.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (in 100 µL final volume). Optimal titration recommended.

Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Ensure instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates and is used under license. Powered by Sirigen™.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, from pro-B cells in bone marrow to mature surface Ig-positive B-cells. Expression is low or absent in plasma cells. CD24 is also found in B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. It may regulate B-cell proliferation, maturation, and autoimmune control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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