Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Brilliant Ultra Violet 395, eBioscience

Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510, 555, 585, 610-30S, 660-40S, 800
• NovaFluor Yellow 570, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, macrophages, and dendritic cells. CD14 binds LPS-LBP complexes with high affinity on monocytes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells. Use at 5 µL (0.5 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).

Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is optimized for cytometers equipped with a 355 nm ultraviolet laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or affiliates, used under license. Powered by Sirigen™.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils. It functions as a lipopolysaccharide receptor and exists in both membrane-bound and soluble forms. CD14 binds LPS via LBP and participates in immune responses, apoptosis recognition, and inflammatory regulation. Multiple transcript variants encode the same isoform. CD14 dysfunction is associated with mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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