Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Ultra Violet 395, eBioscience
마우스 CD11c 단백질을 인식하는 N418 단클론 항체로, Brilliant Ultra Violet 395 형광체로 표지되어 있습니다. 유세포 분석(Flow Cytometry)에 적합하며, 4°C 어둠에서 보관합니다. 고순도의 Affinity Chromatography 정제 항체로, BSA 및 sodium azide를 포함한 PBS 용액 형태입니다.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Ultra Violet™ 395, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | Brilliant Ultra Violet™ 395 |
| Excitation/Emission Max | 347 / 399 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925253 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 605, 650, 711
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- NovaFluor Blue 510, 610-30S, 660-40S, 690
- NovaFluor Yellow 570
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 780
- Request custom conjugation
Product Specific Information
The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX). CD11c forms a heterodimer with beta2 integrin (CD18), expressed on dendritic cells, subsets of intestinal intraepithelial lymphocytes, and some activated T cells. It binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Validated in mouse splenocytes at ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
- Fluorochrome Info: Brilliant Ultra Violet™ 395 (BUV395), excitation 350 nm, emission 395 nm, for UV (355 nm) laser instruments.
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize nonspecific interactions.
Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
CD11, together with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
- CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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