Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MP6-XT22), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MP6-XT22), Brilliant Violet 786, eBioscience

Applications

• Flow Cytometry (Intracellular staining)

Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4(+) T cells. TNF alpha has cytolytic activity against tumor cells and plays a key role in immune regulation. It forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes using Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended ≤ 0.25 µg/test (100 µL final volume).

Fluorochrome: Brilliant Violet™ 786 (BV786) emits at 786 nm and is intended for use with violet (405 nm) laser-equipped cytometers. Protect from light due to photo-induced oxidation sensitivity.

Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. Not compatible with methanol-based fixation.

Recommended Buffers:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. Secreted mainly by macrophages, it binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors. It regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha forms multimers and shows a single 17 kDa band under non-reducing SDS-PAGE. It contributes to tumor necrosis, immune responses to pathogens, and inflammation-related diseases such as autoimmune disorders, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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