Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MP6-XT22), Brilliant Violet 786, eBioscience
Mouse TNF alpha 인식하는 MP6-XT22 클론 단일클론 항체로 Brilliant Violet 786 형광체 결합. Flow cytometry용으로 0.25 µg/test 권장. 405 nm 레이저 사용, 786 nm 방출. 세포 내 염색 및 면역조절 연구에 적합.
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Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MP6-XT22), Brilliant Violet 786, eBioscience
Applications
- Flow Cytometry (Intracellular staining)
Tested Dilution: 0.25 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Violet™ 786, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MP6-XT22 |
| Conjugate | Brilliant Violet™ 786 |
| Excitation / Emission Max | 406 / 788 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_3093252 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 561
- Alexa Fluor 660
- APC
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 650
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4(+) T cells. TNF alpha has cytolytic activity against tumor cells and plays a key role in immune regulation. It forms dimers and trimers and also exists as a 26 kDa membrane-bound form.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes using Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended ≤ 0.25 µg/test (100 µL final volume).
Fluorochrome: Brilliant Violet™ 786 (BV786) emits at 786 nm and is intended for use with violet (405 nm) laser-equipped cytometers. Protect from light due to photo-induced oxidation sensitivity.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. Not compatible with methanol-based fixation.
Recommended Buffers:
- Super Bright Complete Staining Buffer (Product # SB-4401-42)
- Brilliant Stain Buffer™ (Product # 00-4409-75)
Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. Secreted mainly by macrophages, it binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors. It regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha forms multimers and shows a single 17 kDa band under non-reducing SDS-PAGE. It contributes to tumor necrosis, immune responses to pathogens, and inflammation-related diseases such as autoimmune disorders, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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