Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), Brilliant Violet 786, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular Staining)
• Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Brilliant UV 615
• Brilliant Violet 421
• Brilliant Violet 480
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine, and functions as a neutralizing antibody. Mouse IL-4 (also known as BCDF, BCGF-1, BSF-1) is secreted by activated Th2 and NK T cells, and promotes B cell proliferation and differentiation. It induces Ig isotype switching and upregulation of MHC class II and CD23.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on mouse Th2-polarized splenocytes using Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended ≤0.25 µg/test (100 µL final volume). Optimize titration for best results.

Brilliant Violet™ 786 (BV786):

• Tandem dye emitting at 786 nm for use with violet (405 nm) laser.
• Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to reduce non-specific polymer interactions.
• Light sensitive; protect from light.
• Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in dark.
• Not compatible with methanol-based fixation.
• Excitation: 407 nm; Emission: 786 nm; Laser: Violet.

Trademark Notice:
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Interleukin-4 (IL-4) is a pleiotropic cytokine produced by activated T cells, acting as a ligand for IL-4 receptor. It shares overlapping functions with IL-13 and is involved in immune regulation via STAT6 signaling. IL-4 gene is located on chromosome 5q near IL13 and IL5, coordinated by long-range regulatory elements. IL-4 delta 2 is a known splice variant.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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