Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Brilliant Violet 786, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: ≤ 0.25 µg/test

Species Reactivity

Mouse, Rat

Product Specifications

Available Formats

• Alexa Fluor 488
• APC
• Biotin
• Brilliant UV 395 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 650 / 711
• eFluor 450 / 506 / 660
• FITC
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Custom conjugation available

Product Specific Information

The eBio17B7 antibody reacts with mouse and rat IL-17A, without cross-reactivity to IL-17F. IL-17A is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory diseases such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.

This antibody is validated for intracellular staining followed by flow cytometric analysis of mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended use is ≤ 0.25 µg per test (100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test; titration is recommended for optimal performance.

Brilliant Violet™ 786 (BV786) emits at 786 nm and is designed for cytometers equipped with a violet (405 nm) laser. Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) when combining multiple polymer dye-conjugated antibodies to minimize non-specific interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses. It is a 32 kDa disulfide-linked homodimer and part of the IL-17 family (IL-17A–F). IL-17A signals through IL-17R/CDw217, enhancing ICAM-1 expression and stimulating secretion of IL-6, IL-8, G-CSF, and PGE2. It supports CD34+ progenitor proliferation and neutrophil maturation and activates NF-κB and MAPK pathways, increasing COX-2 and nitric oxide production.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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