Thermo Fisher Scientific FOXJ1 Monoclonal Antibody (2A5), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1-10 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 62 publications 62 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-10 µg/mL

View 6 publications 6 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 33 publications 33 publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

ChIP assay (ChIP)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fruit fly, Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2A5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865539

Product Specific Information

Description: This 2A5 monoclonal antibody reacts with Foxj1, a 50-kDa member of the Forkhead-box (FOX) family of winged-helix transcription factors. Expression of Foxj1 is high in ciliated epithelial cells and low in naive T and B cells, where cellular activation results in downregulation of the factor. Foxj1 modulates germinal center formation and Th1 activation by repressing NFkappaB through IkappaBbeta induction. As such, this transcription factor has been suggested to antagonize autoimmune reactions such as systemic lupus erythematosus and rheumatoid arthritis. In addition, Foxj1 has been linked to the formation of motile cilia in epithelial cells.

Applications Reported: This 2A5 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This 2A5 antibody has been tested by western blot of mouse tracheal epithelial cells and testes, as well as by immunohistochemistry of mouse lung tissue. This antibody can be used between 1-10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Forkhead-box J1 (FOXJ1) is a 421-amino acid transcription factor that suppresses T cell activity and spontaneous autoimmunity, through the repression of NF B activity. FOXJ1 also inhibits the humoral immune response in B cells. FOXJ1 deficiency in B cells results in spontaneous and accentuated germinal center formation and is implicated in the development of pathogenic autoantibodies and accentuated responses to immunizations. Abnormal expression of FOXJ1 may be associated with autoimmune diseases and/or other inflammatory diseases. FOXJ1 is also required for cilia formation and left-right axis determination because it increases calpastatin expression, a protein necessary for the ability of basal bodies to anchor to the apical cytoskeleton. FOXJ1 expression may function as an early marker of epithelial cell differentiation, recovery, and function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.