Thermo Fisher Scientific FOXJ1 Monoclonal Antibody (2A5), eBioscience
FOXJ1 단백질을 표적하는 2A5 클론의 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC 등 다양한 분석에 사용 가능. 인간 및 마우스 반응성. 0.5 mg/mL 농도의 액상 형태로 제공되며, PBS(pH 7.2)와 0.09% sodium azide 포함. 연구용으로만 사용.
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Thermo Fisher Scientific FOXJ1 Monoclonal Antibody (2A5), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–10 µg/mL | View 6 publications |
| Immunohistochemistry (IHC) | – | View 62 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–10 µg/mL | View 6 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | View 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 5 publications |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 33 publications |
| Flow Cytometry (Flow) | – | View 2 publications |
| ChIP assay (ChIP) | – | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Fruit fly, Human, Mouse, Pig, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2A5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865539 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
This 2A5 monoclonal antibody reacts with Foxj1, a 50-kDa member of the Forkhead-box (FOX) family of winged-helix transcription factors. Foxj1 is highly expressed in ciliated epithelial cells and low in naive T and B cells, where activation leads to downregulation. It modulates germinal center formation and Th1 activation by repressing NFκB through IκBβ induction, potentially antagonizing autoimmune reactions like systemic lupus erythematosus and rheumatoid arthritis. Foxj1 is also linked to motile cilia formation in epithelial cells.
Applications Reported: Western blotting, immunohistochemistry (formalin-fixed paraffin embedded tissue), and immunocytochemistry.
Applications Tested: Western blot (mouse tracheal epithelial cells and testes), IHC (mouse lung tissue). Recommended concentration: 1–10 µg/mL, titrate for optimal performance.
Target Information
Forkhead-box J1 (FOXJ1) is a 421-amino acid transcription factor that suppresses T cell activity and spontaneous autoimmunity by repressing NFκB activity. It also inhibits humoral immune responses in B cells. FOXJ1 deficiency results in spontaneous germinal center formation and pathogenic autoantibody development. Abnormal FOXJ1 expression is associated with autoimmune and inflammatory diseases. FOXJ1 is essential for cilia formation and left-right axis determination, increasing calpastatin expression required for basal body anchoring. It may serve as an early marker of epithelial cell differentiation and recovery.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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