Thermo Fisher Scientific FGF2 Polyclonal Antibody, PeproTech
Rabbit polyclonal antibody against human FGF2 for WB, ELISA, blocking, and immunostaining applications. High specificity and sensitivity with antigen affinity purification. Suitable for detecting FGF2 in various assays. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 4 publications |
| ELISA | 0.5–2.0 µg/mL | – |
| Blocking Assay (BLOCK) | – | View 1 publication |
| In vitro Assay (IV) | – | View 2 publications |
| Immunostaining (IS) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived Recombinant Human FGF-basic (154 a.a.) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929681 |
Product Specific Information
AA Sequence of recombinant protein:
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-basic. The anti-Human FGF-basic-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-basic matrix.
Sandwich ELISA:
To detect Human FGF-basic by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human FGF-basic (500-P18Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human FGF-basic.
Western Blot:
To detect Human FGF-basic by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human FGF-basic is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization.
FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24, and 34 kDa isoforms are nuclear and may signal independently of transmembrane receptor pathways.
In humans, the gene is located on the q arm of chromosome 4. FGF2 is involved in processes such as limb and nervous system development, wound healing, and tumor growth.
The mRNA for FGF2 contains multiple polyadenylation sites and is alternatively translated from non-AUG and AUG initiation codons, resulting in five isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus (intracrine effect), whereas the AUG-initiated form is cytosolic (paracrine and autocrine effects).
Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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