Thermo Fisher Scientific c-Jun Monoclonal Antibody (4H9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4H9

Immunogen

Purified recombinant fragment of human c-Jun expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11155189

Product Specific Information

MA5-15889 targets c-Jun in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human and mouse samples.

The MA5-15889 immunogen is purified recombinant fragment of human c-Jun expressed in E. Coli.

MA5-15889 detects c-Jun which has a predicted molecular weight of approximately 43kDa.

Target Information

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5-TGA\[CG\]TCA-3. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.