Thermo Fisher Scientific MMP9 Monoclonal Antibody (5C3)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA5-15888 targets MMP9 in indirect ELISA, FACS, and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human MMP9 expressed in E. coli.
MA5-15888 detects MMP9, which has a predicted molecular weight of approximately 92 kDa.

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a zinc and calcium-dependent endopeptidase that degrades extracellular matrix proteins.
It is secreted as a 92 kDa zymogen, and cleavage produces an active enzyme of 82 kDa.
MMP9 contains:

• A gelatin-binding domain (three fibronectin type II units)
• A catalytic domain with zinc-binding site
• A proline-rich type V collagen-homologous domain
• A hemopexin-like domain

MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, and endothelial cells.
It plays roles in inflammatory responses, tissue remodeling, wound healing, tumor growth, and metastasis.
It contributes to skin carcinogenesis and degrades type IV and V collagens.
Studies suggest involvement in IL-8-induced mobilization of hematopoietic progenitor cells and tumor-associated tissue remodeling.
Elevated MMP9 levels are associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.