Thermo Fisher Scientific EOMES Monoclonal Antibody (Dan11mag), PE, eBioscience

Thermo Fisher Scientific EOMES Monoclonal Antibody (Dan11mag), PE, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• eFluor 450
• eFluor 660
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor homologous to T-bet, essential for trophoblast development and gastrulation in vertebrates.
In the immune system, Eomes regulates differentiation of effector and memory CD8+ T cells and NK cells, correlating with high CD122 expression (IL-2R and IL-15R common β-chain).

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Intracellular flow cytometric analysis of mouse splenocytes using Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Refer to BestProtocols® for detailed staining procedure (Protocol B: One-step protocol for intracellular (nuclear) proteins).

• Recommended usage: ≤ 0.5 µg per test (100 µL final volume)
• Cell number range: 10⁵–10⁸ cells/test (empirical optimization recommended)
• Excitation: 488–561 nm; Emission: 578 nm
• Compatible lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

TBR2 belongs to the conserved T-box protein family sharing a common DNA-binding domain. T-box genes encode transcription factors that regulate developmental processes.
TBR2 is crucial for trophoblast development and gastrulation, and plays a key role in brain development, specifically in the specification and proliferation of intermediate progenitor cells and their progeny in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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