Thermo Fisher Scientific MEK2 C-Term Monoclonal Antibody (12A6.G1.G11), FITC
MEK2 단백질의 C-말단을 인식하는 FITC 결합 단일클론 항체로, 인간·마우스·랫트 반응성. Western blot 및 ELISA에 사용 가능. 고순도 Protein A 정제, 안정한 형광 특성(498/517 nm). 연구용으로만 사용.
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Thermo Fisher Scientific MEK2 C-Term Monoclonal Antibody (12A6.G1.G11), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12A6.G1.G11 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | 4°C (short term), -20°C (long term), avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats Available
- Unconjugated
- Biotin
- DyLight 680
- DyLight 800
- HRP
- Custom conjugation available upon request
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if solution is not clear after standing.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
- Cross-reactivity expected with human, mouse, and rat.
- Does not react with MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase involved in mitogen growth factor signal transduction.
MEK2 activates ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif in the activation loop.
MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222.
Threonine 292 of MEK1 is phosphorylated by ERK2, forming a negative feedback loop that suppresses MEK1 activation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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