Thermo Fisher Scientific ERK2 Internal Monoclonal Antibody (3H9.A1.A8), FITC
ERK2 내부 영역을 인식하는 FITC 결합 단일클론 항체로, 인간·마우스·랫트에 반응합니다. Western blot 및 ELISA에 적합하며, 단백질 A로 정제된 고순도 제품입니다. 형광 분석용으로 498/517 nm에서 최적의 신호를 제공합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1 µg/mL
ELISA
- Tested Dilution: 1:40,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3H9.A1.A8 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- Biotin
- DyLight 680
- DyLight 800
- HRP
- Custom conjugation available
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK1 isoform.
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), serve as integration points for multiple biochemical signals and participate in cellular processes such as proliferation, differentiation, transcription regulation, and development. Activation requires phosphorylation by upstream kinases, after which ERK2 translocates to the nucleus to phosphorylate nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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