Thermo Fisher Scientific 6X His Tag Monoclonal Antibody (33D10.D2.G8), HRP
6X His 태그 단백질 검출용 HRP 결합 단클론 항체. Western blot, ELISA, IHC, IP 등 다양한 응용에 적합. 높은 특이성과 비교차 반응성으로 태그 단백질 확인에 유용. 단기 4°C, 장기 -20°C 보관.
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Thermo Fisher Scientific 6X His Tag Monoclonal Antibody (33D10.D2.G8), HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:1,000–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 33D10.D2.G8 |
| Immunogen | Produced in mice by repeated immunizations with 6X His epitope tag peptide (H-H-H-H-H-H) conjugated to KLH using maleimide. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15 M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
- Unconjugated
- Atto 550
- Atto 647N
- Biotin
- Cyanine3
- Cyanine5
- DyLight 405
- DyLight 488
- DyLight 549
- DyLight 649
- DyLight 680
- DyLight 800
- Request custom conjugation available
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag and is useful for determining its presence in various assays. It reacts with all His-tagged proteins tested and does not cross-react with endogenous bacterial proteins. The antibody recognizes His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-terminus of targeted proteins in transfected or transformed cells.
Target Information
The 6X His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). Commonly expressed at either the N- or C-terminal region of recombinant proteins, it enables purification via immobilized metal affinity chromatography (IMAC). Epitope tagging allows detection and purification of recombinant proteins for which no specific antibody exists. His-tagged proteins can be purified using Ni²⁺-NTA adsorbents due to the strong and selective affinity between histidine clusters and Ni²⁺ ions. His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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