Thermo Fisher Scientific CD309 (FLK1) Recombinant Rabbit Monoclonal Antibody (SU03-42)
Thermo Fisher의 CD309 (FLK1) 재조합 토끼 단클론 항체(SU03-42)는 인간 VEGF Receptor 2를 인식하며, WB, IHC, ICC/IF에 적합합니다. 높은 특이성과 일관된 로트 간 재현성을 제공하며, 단백질 A로 정제된 액상 형태로 공급됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SU03-42 |
| Immunogen | Recombinant protein within Human VEGF Receptor 2 (aa 30–630) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term); for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809411 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase that binds vascular endothelial growth factor (VEGF) with high affinity in vitro. It is expressed early in development by endothelial cell precursors.
FLK1 (VEGF Receptor 2) encodes one of the two receptors of VEGF, known as kinase insert domain receptor. Its signaling and trafficking are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin αVβ3, and T-cell protein tyrosine phosphatase.
Mutations in FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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