Thermo Fisher Scientific Phospho-Nrf2 (Ser40) Recombinant Rabbit Monoclonal Antibody (SU0334)
Phospho-Nrf2 (Ser40) 인산화 단백질을 검출하는 재조합 토끼 단일클론 항체. Western blot, IHC, ICC, Flow cytometry에 적합. 높은 특이성과 재현성 제공. 동물 유래 성분이 없는 포뮬레이션으로 일관된 품질 보장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1:50–1:100 | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SU0334 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser40 of Human Nrf2 (aa 20–69) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809408 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Nrf1 (also designated TCF11), Nrf2, and Nrf3 are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE (Maf recognition elements) sequences.
Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from the repressor protein Keap1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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