Thermo Fisher Scientific Phospho-AKT1 (Ser473) Recombinant Rabbit Monoclonal Antibody (12H9L7)
Phospho-AKT1 (Ser473)를 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 민감도를 제공. Human, Monkey, Rat에 반응 예측됨. 액상 형태로 0.5 mg/mL 농도. 세포 생존 및 신호전달 연구용으로 적합.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 12H9L7 |
| Immunogen | Peptides corresponding to human Phospho-AKT1 (Ser473) [aa468–aa477] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2784574 |
Product Specific Information
This antibody is predicted to react with Human, Monkey, and Rat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells.
This protein kinase is activated by insulin, PI3K, IGF1, and other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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