Thermo Fisher Scientific FOXO1/FOXO3/FOXO4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human FOXO1(Accession Q12778), corresponding to amino acid residues M1-A49. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FOXO1/FOXO3/FOXO4,uniProtId:Q12778-1,ncbiNodeId:9606,antigenRange:1-49,antigenLength:655,antigenImageFileName:PA5-104262_FOXO1FOXO3FOXO4_Q12778-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104262_FOXO1FOXO3FOXO4_Q12778-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:FOXO1/FOXO3/FOXO4,uniProtId:P98177-1,ncbiNodeId:9606,antigenRange:1,antigenLength:505,antigenImageFileName:PA5-104262_FOXO1FOXO3FOXO4_P98177-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104262_FOXO1FOXO3FOXO4_P98177-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:FOXO1/FOXO3/FOXO4,uniProtId:O43524-1,ncbiNodeId:9606,antigenRange:1,antigenLength:673,antigenImageFileName:PA5-104262_FOXO1FOXO3FOXO4_O43524-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104262_FOXO1FOXO3FOXO4_O43524-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2853576

Product Specific Information

Antibody detects endogenous levels of total pan-FOXO1/3/4.

Target Information

FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors. FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined.FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.