Thermo Fisher Scientific Phospho-SF1 (Ser80, Ser82) Polyclonal Antibody
SF1 단백질의 Ser80 및 Ser82 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 및 IHC(Paraffin)에서 검증됨. 인산화된 SF1의 내인성 수준만 검출. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SF1 (Accession Q15637), corresponding to amino acid residues around phosphorylated Ser82. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816012 |
Product Specific Information
This antibody detects endogenous levels of SF1 only when phosphorylated at Ser80/82.
Target Information
Splicing factor 1 (SF1) is a nuclear pre-mRNA splicing factor that specifically recognizes the intron branch point sequence at the 3′ splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains:
- An N-terminal U2AF ligand motif
- A central hnRNP K homology motif and quaking 2 region that binds a key branch-site adenosine
- A zinc knuckles domain
- A C-terminal proline-rich domain
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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