Thermo Fisher Scientific H3K18ac Recombinant Rabbit Monoclonal Antibody (RM166)
Histone H3의 Lys18 아세틸화(K18ac)에 특이적으로 반응하는 재조합 토끼 단클론 항체. Western blot, IHC, ICC, ELISA, ChIP 등 다양한 응용에 적합. 높은 특이성과 로트 간 일관성 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | - |
| Immunohistochemistry (IHC) | 1–10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 0.5–5 µg/mL | - |
| ELISA | 0.5–2 µg/mL | - |
| ChIP assay (ChIP) | 2–10 µg/mL | View 1 publication |
| Luminex (LUM) | 0.1–0.5 µg/mL | - |
| Peptide Array (Array) | 0.25 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM166 |
| Immunogen | Acetyl-peptide corresponding to Acetyl-Histone H3 (Lys18) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661900 |
Product Specific Information
This antibody reacts specifically with Histone H3 acetylated at Lysine 18 (H3K18ac) and shows no cross-reactivity with other acetylated lysines in histone H3.
Recombinant rabbit monoclonal antibodies are generated using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Along with other core histones, it forms nucleosomes by binding to DNA. Histones play essential roles in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo various post-translational modifications, including acetylation, methylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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